Abstract
ABSTRACTWe report here the complete genome sequence of Vibrio campbellii, isolated from Penaeus vannamei cultured in a Latin American country. The Tn3-like transposon and pirAB genes were encoded on the plasmid pLA16-2. These data support the geographical variations in the virulence plasmid found among acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio isolates from Latin America and Asia.
Highlights
We report here the complete genome sequence of Vibrio campbellii, isolated from Penaeus vannamei cultured in a Latin American country
The PacBio long-read data (1,787,793,760 bp, 255,476 reads) were de novo assembled by FALCON version 0.2.1, and the Illumina paired-end reads (1,053,525,915 bp, 10,439,662 reads) were mapped to the assembled contigs to improve the accuracy of the genome
The fully assembled genome contained 6,124,368 bp consisting of two chromosomes, designated Chr I (3,579,637 bp) and Chr II (2,230,177 bp), and a total of four plasmids, designated pLA16-1 (109,793 bp), pLA16-2 (73,461 bp), pLA16-3 (67,308 bp), and pLA16-4 (63,992 bp)
Summary
We report here the complete genome sequence of Vibrio campbellii, isolated from Penaeus vannamei cultured in a Latin American country. Sequencing was performed by Macrogen, Inc., using a hybrid approach with the PacBio RS II (Pacific Biosciences) (20-kb SMRTbell template library) and Illumina HiSeq 2000 (pairedend short-read data) platforms. The PacBio long-read data (1,787,793,760 bp, 255,476 reads) were de novo assembled by FALCON version 0.2.1, and the Illumina paired-end reads (1,053,525,915 bp, 10,439,662 reads) were mapped to the assembled contigs to improve the accuracy of the genome.
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