Abstract
Acinetobacter baumannii has emerged as an important nosocomial pathogen causing health care-associated infections. In this study, we determined the genome of a twitching-positive clinical strain, B8300, isolated from a hospital in southern India. De novo assembly of PacBio long-read sequencing data generated the B8300 genome that consists of a chromosome of 3.82 Mbp and a plasmid of 25.15 kbp.
Highlights
Acinetobacter baumannii has emerged as an important nosocomial pathogen causing health care-associated infections
The reads were assembled de novo with Hierarchical Genome Assembly Process 3 (HGAP3) (5) within the singlemolecule real-time (SMRT) Analysis version 2.3.0 software
SMRT Analysis with the default parameters was used for detecting base modifications in the genome
Summary
Acinetobacter baumannii has emerged as an important nosocomial pathogen causing health care-associated infections. A. baumannii is often intrinsically resistant to many antibiotics and has the ability to uptake new resistance genes from the environment, making the infections difficult to treat (2). The organism is classically described as nonmotile; many clinical isolates display both swarming and twitching motilities (3). We have isolated an A. baumannii strain (B8300) from the bloodstream infection of a 29-year-old male patient from Christian Medical College, Vellore, India.
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