Abstract

We report the complete, closed genome sequence and complete methylome of Azospirillum thiophilum strain BV-ST.

Highlights

  • The genome was sequenced using the Pacific Biosciences (PacBio RSII) single-molecule real-time (SMRT) sequencing platform

  • The total genome size was 7,609,460 bp consisting of eight closed chromosomes of 3,037,424, 1,354,909, 878,428, 712,512, 694,460, 518,276, 330,423, and 83,026 bp, respectively, which together encoded a total of 6,620 genes. This multichromosome assembly is consistent with the structure of previously published Azospirillum genome sequences [4,5,6]

  • The assembled sequences were annotated with the NCBI Prokaryotic Genomes Annotation Pipeline (PGAP) and were deposited at DDBJ/EMBL/ GenBank

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Summary

Introduction

The genome was sequenced using the Pacific Biosciences (PacBio RSII) single-molecule real-time (SMRT) sequencing platform. Two SMRTbell libraries were prepared according to modified 10-and 20-kb PacBio sample preparation protocols, including additional separation on a BluePippin DNA size selection system (Sage Science, Beverly, MA) and sequenced using PacBio’s C4 chemistry on 17 SMRT cells using either 180- or 240-min collection times.

Results
Conclusion

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