Abstract
We report the complete, closed genome sequence and complete methylome of Azospirillum thiophilum strain BV-ST.
Highlights
The genome was sequenced using the Pacific Biosciences (PacBio RSII) single-molecule real-time (SMRT) sequencing platform
The total genome size was 7,609,460 bp consisting of eight closed chromosomes of 3,037,424, 1,354,909, 878,428, 712,512, 694,460, 518,276, 330,423, and 83,026 bp, respectively, which together encoded a total of 6,620 genes. This multichromosome assembly is consistent with the structure of previously published Azospirillum genome sequences [4,5,6]
The assembled sequences were annotated with the NCBI Prokaryotic Genomes Annotation Pipeline (PGAP) and were deposited at DDBJ/EMBL/ GenBank
Summary
The genome was sequenced using the Pacific Biosciences (PacBio RSII) single-molecule real-time (SMRT) sequencing platform. Two SMRTbell libraries were prepared according to modified 10-and 20-kb PacBio sample preparation protocols, including additional separation on a BluePippin DNA size selection system (Sage Science, Beverly, MA) and sequenced using PacBio’s C4 chemistry on 17 SMRT cells using either 180- or 240-min collection times.
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