Abstract
A reference preparation for simian immunodeficiency virus (SIV) RNA nucleic acid assays was characterized by complete genome deep sequencing. The entire coding sequence and flanking long terminal repeats, including minority species, were determined. This information will inform SIV research investigations and aid evaluation and development of amplification assays for SIV RNA quantification.
Highlights
A reference preparation for simian immunodeficiency virus (SIV) RNA nucleic acid assays was characterized by complete genome deep sequencing
In cynomolgus macaques (Macaca fascicularis), a high-titer bulk SIV positive plasma stock recovered at the peak of virus replication in vivo was developed into an SIV RNA reference panel (C1ϪC5), based on the SIVmac251/32H/L28 isolate [1, 2]
The genetic composition of this SIV RNA reference material has not been described; here, we report the previously undescribed complete genome sequence using deep sequencing approaches, which are analogous to those previously reported for HIV-1 and HIV-2 [3, 4]
Summary
A reference preparation for simian immunodeficiency virus (SIV) RNA nucleic acid assays was characterized by complete genome deep sequencing. Model systems to evaluate putative vaccine strategies and pathogenesis studies of HIV/AIDS employ strains of simian immunodeficiency virus (SIV) in experimental design. In cynomolgus macaques (Macaca fascicularis), a high-titer bulk SIV positive plasma stock recovered at the peak of virus replication in vivo was developed into an SIV RNA reference panel (C1ϪC5), based on the SIVmac251/32H/L28 isolate [1, 2].
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