Abstract
ABSTRACTWe describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae. The properties of FlHV1 permit assignment to the genus Alphahypovirus in the family Hypoviridae. This is the first report of a mycovirus identified in F. langsethiae.
Highlights
We describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae
The AH32 strain of Fusarium langsethiae was cultured on potato dextrose agar plates overlaid with cellophane membranes for 4 days at 25°C in the dark
Multiple alignments and phylogenetic analyses of the viral polyproteins and the conserved domain (RdRp and Hel) aa sequences using the neighbor-joining method revealed that FlHV1 formed a well-supported phyloge
Summary
We describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae. The AH32 strain of Fusarium langsethiae was cultured on potato dextrose agar plates overlaid with cellophane membranes for 4 days at 25°C in the dark. The mycelium mass was used for dsRNA extraction using the CF-11 cellulose chromatography method (with 16% ethanol concentration).
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