Abstract
ABSTRACTWe report the genetic characterization of a new papillomavirus (HPV_MTS1) isolated and fully cloned from a skin swab. The L1 open reading frame of HPV_MTS1 was 85% identical to its closest human papillomavirus (HPV) type 80, which belongs to the species beta-2 of the genus Betapapillomavirus, hence qualifying it as a new HPV type.
Highlights
We report the genetic characterization of a new papillomavirus (HPV_ MTS1) isolated and fully cloned from a skin swab
The amplified product was digested with EcoRI and cloned into the pUC19 vector for sequencing using the primer-walking strategy (GATC Biotech, Germany) which covered nucleotides 842 to 6,935 (6,094 bp) of the viral genome
A long-range PCR was performed on the rolling circle amplification (RCA) product as a template using TaKaRa LA Taq DNA polymerase (TaKaRa Bio Inc.) and HPV_MTS1 specific primers to obtain the remaining part of the genome
Summary
We report the genetic characterization of a new papillomavirus (HPV_ MTS1) isolated and fully cloned from a skin swab. The complete genome of a new HPV type (HPV_MTS1; 7,405 bp) was obtained by amplifying skin swab DNA using multiply primed rolling circle amplification (RCA) according to the manufacturer’s instructions (Illustra TempliPhi 100 amplification kit, GE Healthcare, Piscataway, NJ). The amplified product was digested with EcoRI and cloned into the pUC19 vector for sequencing using the primer-walking strategy (GATC Biotech, Germany) which covered nucleotides (nt) 842 to 6,935 (6,094 bp) of the viral genome.
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