Abstract
ABSTRACTStreptococcus uberis forms part of the native microbiota of cattle and is able to opportunistically infect the mammary gland; as such, it is a leading cause of bovine mastitis globally. Here, we report the complete genome sequence of S. uberis NZ01, isolated in New Zealand from a cow with a clinical case of bovine mastitis.
Highlights
Streptococcus uberis forms part of the native microbiota of cattle and is able to opportunistically infect the mammary gland; as such, it is a leading cause of bovine mastitis globally
The complete NZ01 genome comprises a single chromosome of 1,863,842 bp, with 1,808 coding DNA sequences (CDSs) and 1,886 predicted genes
146 CDSs found in the NZ01 genome are novel, compared to those of strain 1040J from the United Kingdom
Summary
Streptococcus uberis forms part of the native microbiota of cattle and is able to opportunistically infect the mammary gland; as such, it is a leading cause of bovine mastitis globally. DNA extraction and next-generation sequencing were performed at the Microbiological Diagnostic Unit Public Health Laboratory of the University of Melbourne. Genomic DNA was prepared from a culture grown from a single colony using a JANUS Chemagic workstation and Chemagic DNA/RNA kit (PerkinElmer, USA). DNA libraries were created using the Nextera XT DNA preparation kit (Illumina, USA).
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