Abstract
ABSTRACTA new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratory-scale leach bed biogas system. The draft annotated genome sequence presented in this study and 16S rRNA gene sequence analysis indicated the affiliation of HV4-5-B5C with the family Ruminococcaceae outside recently described genera.
Highlights
A new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratoryscale leach bed biogas system
Analysis based on the average nucleotide sequence identity (ANI) [11] showed closest affiliation to Clostridium sporosphaeroides (68.83% identity), applying ANIBlast, and to Clostridium leptum (88.36% identity), applying ANI-MUMmer
Based on the percentage of conserved proteins (POCP) [9], the closest genome-sequenced relative of the novel Ruminococcaceae bacterium was Clostridium sporosphaeroides (38.31% identity), as indicated by the average amino acid identity (AAI) [10] (54.72% identity)
Summary
A new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratoryscale leach bed biogas system. Genome sequencing was performed using the Illumina NextSeq 500 platform. A Nextera XT library with a mean insert size of around 300 nucleotides (nt) was constructed and sequenced in a combination of 150-bp paired-end (PE) reads. 21,629,863 PE sequences with a mean length of 150.32 nt were obtained.
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