Complete Genome Sequence of a Multidrug-Resistant Salmonella enterica Serovar Typhimurium var. 5− Strain Isolated from Chicken Breast

Maria Hoffmann,Richard J Roberts,Shaohua Zhao,Peter Evans,Jianghong Meng,Justin Payne,Tim Muruvanda,Marc W Allard,Patrick F Mcdermott,Ruth Timme,Eric W Brown,Jonas Korlach

doi:10.1128/genomea.01068-13

Abstract

Salmonella enterica subsp. enterica serovar Typhimurium is a leading cause of salmonellosis. Here, we report a closed genome sequence, including sequences of 3 plasmids, of Salmonella serovar Typhimurium var. 5− CFSAN001921 (National Antimicrobial Resistance Monitoring System [NARMS] strain ID N30688), which was isolated from chicken breast meat and shows resistance to 10 different antimicrobials. Whole-genome and plasmid sequence analyses of this isolate will help enhance our understanding of this pathogenic multidrug-resistant serovar.

Highlights

In this report, we announce the availability of a fully closed genome sequence, including sequences of three plasmids, of Salmonella serovar Typhimurium strain CFSAN001921, which was isolated from chicken breast in New York in 2011
The genomic DNA of strain CFSAN001921 was isolated from overnight cultures using the DNeasy blood & tissue kit (Qiagen Inc., Valencia, CA) and sequenced using the Pacific Biosciences (PacBio) RS sequencing platform
The 10-kb continuous-long-read (CLR) data were de novo assembled using the PacBio hierarchical genome assembly process (HGAP)/ Quiver software package, followed by Minimus 2, and they were polished with Quiver [8]

Summary

Introduction

We announce the availability of a fully closed genome sequence, including sequences of three plasmids, of Salmonella serovar Typhimurium strain CFSAN001921, which was isolated from chicken breast in New York in 2011. The genomic DNA of strain CFSAN001921 was isolated from overnight cultures using the DNeasy blood & tissue kit (Qiagen Inc., Valencia, CA) and sequenced using the Pacific Biosciences (PacBio) RS sequencing platform. We prepared a single 10-kb library that was sequenced using C2 chemistry on 8 single-molecule real-time (SMRT) cells with a 90-min collection protocol on the PacBio RS.

Results

Conclusion