Complete genome sequence data of multidrug-resistant Aeromonas hydrophila Ah27 isolated from intussusception channel catfish (Ictalurus punctatus)

Abstract

Aeromonas hydrophila Ah27, a multidrug-resistant bacterium, was separated from the liver of an intussusception channel catfish (Ictalurus punctatus) in Guizhou province, China. The genome of A. hydrophila Ah27 was sequenced using the PacBio RS and Illumina sequencing platforms. The complete genome of A. hydrophila Ah27 consists of a circular chromosome with 5,102,912 bp and a plasmid with 6162 bp. The chromosome's average GC content is 60.75 %. NCBI Prokaryotic Genome Annotation Pipeline (v1.02) was used to annotate the genome with the default parameters. The results showed that the chromosome contained 4726 coding genes, including 4505 protein coding sequences, 119 tRNA genes, 31 rRNA genes, 7 ncRNAs and 64 Pseudo Genes. According to the annotation analysis, strain Ah27 encodes β-lactams, aminoglycosides, quinolones, macrolides, sulfonamides, tetracyclines resistance genes, and four significant types of efflux pump genes. The Kirby-Bauer disk method was used to detect the sensitivity or resistance of Ah27. The results showed that strain Ah27 was resistant to 27 antibiotics, including spectinomycin, streptomycin, enrofloxacin, tetracycline, bacitracin, clarithromycin, erythromycin, trimethoprim, etc. At the same time, 30 mobile genetic elements were also obtained by comparison with the MobileElementFinder tool. The complete genome sequence of A. hydrophila Ah27 may also provide a fresh perspective on disease prevention and antimicrobial resistance mechanisms.