Abstract
ABSTRACTNassella hyalina (cane needle grass) is on the Alert List for Environmental Weeds in Australia. We present here the first complete chloroplast sequence of N. hyalina reconstructed from Illumina whole-genome sequencing. The complete chloroplast sequence is 137,606 bp in size and has a gene content and structure similar to those of other published chloroplast genomes of Stipeae.
Highlights
Nassella hyalina is on the Alert List for Environmental Weeds in Australia
DNAs that met the quality control (QC) requirements were used for library construction (500-bp insert size) before being sequenced on an Illumina HiSeq 2000 sequencer to generate 125-bp paired-end reads (BGI, Hong Kong)
NOVOPlasty (3), an algorithm developed for the de novo assembly of mitochondrial and chloroplast genomes from wholegenome data, was applied to extract chloroplast genome sequences from the raw reads of the FASTQ files
Summary
Nassella hyalina (cane needle grass) is on the Alert List for Environmental Weeds in Australia. A modified cetyltrimethylammonium bromide (CTAB) protocol (2) was applied to extract total genomic DNA from fresh leaves of the specimen. DNAs that met the quality control (QC) requirements were used for library construction (500-bp insert size) before being sequenced on an Illumina HiSeq 2000 sequencer to generate 125-bp paired-end reads (BGI, Hong Kong).
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