Year-end Sale % OFF 
Abstract
Mucoid (MTB313) and nonmucoid (MTB314) strains of group A streptococcus emm type 1 were simultaneously isolated from a single patient suffering from streptococcal meningitis. Whole-genome sequencing revealed that MTB313 carried a nucleotide substitution within rocA, which generated an amber termination codon.
Highlights
We identified mucoid (MTB313) and nonmucoid (MTB314) strains of group A streptococcus (GAS) emm type 1 at the same time on the same blood agar plate with application of a cerebrospinal fluid specimen of a 70-year-old Japanese woman suffering from streptococcal meningitis
We performed comparative genomics based on whole-genome sequencing of both GAS strains to comprehensively identify the naturally occurring nucleotide substitutions
The sequences from MTB313 and MTB314 were analyzed for the detection of local and structural variations compared to those of MGAS5005 (1) by using the GS Reference Mapper application
Summary
We identified mucoid (MTB313) and nonmucoid (MTB314) strains of group A streptococcus (GAS) emm type 1 at the same time on the same blood agar plate with application of a cerebrospinal fluid specimen of a 70-year-old Japanese woman suffering from streptococcal meningitis. We performed comparative genomics based on whole-genome sequencing of both GAS strains to comprehensively identify the naturally occurring nucleotide substitutions. The sequences from MTB313 and MTB314 were analyzed for the detection of local and structural variations compared to those of MGAS5005 (a virulent type strain of emm1 GAS) (1) by using the GS Reference Mapper application (analysis software included with the 454 system).
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
