Comparation of microcolumn gel test and tube anti-globulin test with IgG anti A(B)of pregnant women

Abstract

Objective Compared study on microcolumn gel test(MGT)and tube anti-globulin test(TAT)in the detection of pregnant women IgG anti A(B)in the application of antibody and evaluate the application value of MGT in the prediction of HDN. Methods Choosed blood samples of 443 cases of O blood type pregnant women whose husband were not O blood type as the research object. Every specimen were tested by MGT method and TAT method, and the data were treated statistically. Results The positive rate of MGT method and TAT method were: 30%and 12.5% which had statistical significance(χ2=15.95,P<0.05). The difference was significant in positive cases titer distribution(t=15.13,P<0.01). Conclusion The micro column gel method is rapid, simple, sensitive and repeatable compared of tube anti-globulin test(TAT). Key words: IgG anti A(B); Microcolumn gel test; Tube anti-globulin test