Clinical Significance of the detection of HBV-DNA by the Real-time Fluorescence quantitative PCR

Abstract

Objective To analyze HBV-DNA results of hepatitis B patients using real-time fluorescence quantitative PCR detection and to explore the significance of their clinical treatment. Methods 850 cases of clinical specimens were detected by time-resolved fluorescence immunoassay quantitative determination and fluorescent quantitative PCR assay detected HBV-DNA of blood samples and the results were divided groups. Results There were 261 positive in group that the HBsAg( + ), HBeAg ( + )and HBcAb ( + )were positive and positive rate was 86.4% (261/302)and its quantitative PCR for copy number was(2. 21 ± 0. 76) × 107/ml.There were 96 positive in group that the HBsAg( + ) ,HBeAb( + )and HBcAb( + )were positive and the positive rate was 29.91% (96/321)and its quantitative PCR for copy number was( 1.69 ± 0. 98) × 106/ml. There were 9 were positive in group that the HBsAg( + )and HBcAb( + )were positive and positive rate was 28. 13% (9/32). There were 4 positive in group that the HBeAb ( + ) and HBcAb ( + ) were positive and positive rate was 14. 29% (4/28). There were 6 positive in group that the HBcAb( + )was positive and the positive rate was 42.9% (6/11). There were 5 positive in group that the HBsAb( + ), HBeAb( + ) and HBcAb( + ) were positive and the positive rate was 6. 75% (5/74). There were 2 positive in group that HBsAb( + )and HBeAb( + )positive and the positive rate was 14. 3% (2/13). There were 1 positive in group that HBsAb( + ) and HBcAb ( + )were positive and a positive rate was 7.69% (1/12). 4 were negative in group of HBsAb( + ). There were 21 positive in the group that were HBsAg( + ), HBsAb( + ), HBeAg( + )and HBcAb( + )were positive and the positive rate was 87.50% (21/24). The positive rate was 100% 1 in group of HBsAg( + ). There were 3 positive in group that HBsAg( + ), HBsAb ( + ), HBeAb ( + ) and HBcAb ( + ) were positive and the positive rate was 30. 00% (3/10). The remaining 38 patients were negative results. Conclusion PCR quantitative determination of HBV-DNA can be a true reflection of hepatitis B virus infection and replication in vivo and more conducive to clinical treatment and efficacy. Key words: Hepatitis B Serum Markers; Time-resolved Fluorescence Immunoassay; Fluorescence Quantitative PCR Technique; Clinical Application