Use the methods of Real-time fluorescence quantitative PCR to detect the HBV DNA content in the patients serum froth 312 cases of the hepatitis B pstients

Abstract

Objective To investigate the characteristics of serum HBV DNA content through detecting the diffent serum markers of hepatitis B using the method of the real-time fluorescence quantitative PCR.Methods 512 cases of hepatitis B serum markers were detected with ELISA, and simultaneously use the real-time fluorescence quantitative PCR to detect HBV DNA content in the serum. Statistics for each group of HBV DNA-positive cases and its positive rate (%) were separately done.u test were used for the statistical treannent.Results HBsAg (+) / HBeAg (+) / anti-HBc (+) group has 168 cases, HBV DNA positive has 61 cases (95.8%); HBsAg (+) /anti-HBe (+) /anti-HBc (+) group has 163 cases,HBV DNA positive has 51 cases (31.3%); HBsAg (+) / anti-HBc (+) group has 181 cases,HBV DNA positive has 94 cases (51.9%). Comparisons among three.groups were significantly different (P<0.001).Conclusions The reproduction of HBV in the HBsAg (+)/ HBeAg (+)/ anti-HBc (+) group were the most active.Real-time fluorescence quantitative PCR detection of HBV DNA is the better method to reflect the existence and the reproduction of hepatitis B virus than Serological markers. Key words: Real-time fluorescence quantitative PCR; HBV DNA; Hepatitis B virus; Serological markers