Analysis of changes in viral load of different course of disease and main indexes of liver function and immune function in HBV infection patients

Abstract

Objective To explore the changes of main indexes of liver function of peripheral blood, T cell subsets and the viral load in hepatitis B virus infection patients in different course and its clinical significance. Method 170 cases of hepatitis B virus infection patients were divided into acute hepatitis B group (18 cases), chronic hepatitis B group (73 cases), liver cirrhosis group (41 cases) , hepatic carcinoma group (38 cases), and normal control group of 40 cases according to the course. The related indexes of liver function and T cell subsets were detected by the automatic biochemical analyzer and flow cytometry, and the content of HBV-DNA was detected by real-time fluorescent quantitative PCR method. Results Alanine transaminase (ALT) and aspartate transaminase (AST) increased in four experimental groups compared to normal controls, with significant difference (P 0.05). The increase of alkaline phosphatase(ALP) was in liver cancer patients, the levels of ALP in chronic hepatitis B was low. Groups of total protein (TP) were within the normal reference range.CD3+ T cells in peripheral blood of the four experimental groups were lower than in the normal controls, the patients with hepatic carcinoma and cirrhosis was obviously lower. The proportions of CD4+ T cells rose in acute hepatitis group, but the percentage of CD8+ T cells is the lowest in acute hepatitis B patients.The most obvious rise of HBV DNA virus load in peripheral blood was in chronic hepatitis B group, but the HBV-DNA level is relatively low in acute hepatitis, liver cirrhosis and liver cancer patients, the lowest was in acute hepatitis B, and the differences were all statistically significant(P<0.05)compared with other groups. Conclusion The liver function, the expression of T cell subsets and HBV-DNA content had significant difference in hepatitis B virus infection patients with different course, the combined detection of these projects can provide guidance for the clinical assessment of the disease and course, and provide reliable basis for judging the prognosis of the disease and treatment. Key words: Hepatitis B virus; Liver function; HBV-DNA; T lymphocyte subsets