Clinical analysis of urine bacteria culture in children with urolithiasis

Abstract

Objective To study the correlation between age, gender, stone size, bilateral or unilateral urinary calculi, anatomical location of calculi and the positive rate of urine culture, and to investigate the pathogenic bacteria and drug resistance in children with urolithiasis. Methods Files of patients from department of urology in People′s Hospital of Xinjiang Uygur Autonomous Region from January 2013 to March 2017 were reviewed. The observed indexes included age, gender, stone size, bilateral or unilateral renal involvement, anatomical location of calculi, and the results of urine culture and drug susceptibility test.Only those patients with calculi and less than 12 years were studied.All files of patients with documented urological procedures prior to urinary tract infection occurrence were excluded from the study. CT technique was used to determine stone size and location.Collected urine samples were screened for urinary tract infection. Results A total of 107 patients were studied. The mean age was (4.37±2.97) years.Seventy-four cases (69.2%) were males; 33 cases(30.8%) were females. Urinary tract infection was found in 27 cases(25.2%). The most common pathogenic bacteria found on our culture plate of urinary tract infection positive patients was Escherichia coli (48.1%; 13/27). Gram negative bacteria were most sensitive to imipenem(100.0%; 24/24), cefoxitin (88.2%; 15/17), and Piperacillin/Tazobactam(87.5%; 21/24). Gram negative bacteria had a high resistance rate to ampicillin (84.2%; 16/19), cefazolin(81.0%; 17/21), cefuroxime(80.0%; 16/20), TMP-SMX(77.3%; 17/22)and ceftriaxone(73.9%; 17/23) in our center. Conclusions There was no association between these parameters(age, sex, stone size, bilateral or unilateral renal involvement, and anatomical location of calculi) and positive rate of urine culture in children with urolithiasis. Escherichia coli was the most common causative agent of urinary tract infection in children with urolithiasis.Gram negative bacteria had a high resistance rate to ampicillin, cefazolin, and cefuroxime in our center. Key words: Child; Urinary tract infections; Urinary calculi; pathogenic bacteria; antimicrobial resistance