Analysis on distriution and drug resistance of pathogenic bacteria by midstream urine culture among 1 348 cases

Abstract

Objective To investigate the distribution and drug resistance of microbial pathogens in midstream specimen of urine of patients with urinary tract infection, provide reference and basis for clinical medication. Methods A total of 1 348 urine specimens were in this study. These specimens were collected from more than 500 basic level hospitals in June 2015 to May 2016. These hospitals distributed in 13 cities of Jiangsu province. The urine specimens were cultured on blood agar and MacConkey Agar respectively. VITEK COMPACT automatic microbial analysis system and API identification strip for bacterial identification were utilized. Strain identification were carried out when the isolated gram negative bacteria was more 105 cfu/ml and gram positive bacteria was more than 104 cfu/ml. Drug sensitivity test results were determined by CLSI(2015). Results Microbial pathogens were isolated from 450 specimens accounted 33.38% of the all (450/1 348). Three hundred fifty-eight strains of gram negative bacteria was isolated, account for 79.56% of all strains (358/450). Seventy-six strains of gram positive bacteria were isolated. Sixteen strains of fungi were also isolated, only account for 3.56% of all (16/450). Drug sensitivity test showed that the resistance rates of E. coli to ampicillin, piperacillin, cefazolin, cefuroxime were 84.16%, 80.37%, 87.12% and 93.98% respectively; the resistance rates of Enterococcus to tetracycline, rifampin, quinupristin-dalfopristin, minocycline were 81.40%, 86.67%, 100% and 83.33% respectively. Conclusions E.coli and Enterococcus faecalis are the the main pathogens of urinary tract infections. Drug resisitance to penicillin, cephalosporins, tetracyclines and rifamycin is greater. These results provide instructional significance to rational use of antimicrobial agents Key words: Urinary tract infection; Urine culture; Pathogens; Durg resistance