Chromatographic Separations and Analysis: Chiral Separations by Thin Layer Chromatography

Abstract

Racemate separation can be divided into two types. The first type is the direct method of separation and the second is an indirect method. The direct type of separation includes the application of a chiral stationary phase (cellulose and its derivatives, chiral ligand-exchange chromatography-system or silica), a stationary phase impregnated with a chiral selector (enantiomers of various chiral compounds, e.g., acids, alkaloids, amino acids, and their derivatives) or chiral mobile phase additives (e.g., various cyclodextrins and their derivatives, alkaloids). The principle of the second type of separation consists of a reaction between racemate and a chiral derivatizing reagent, which yields separate diastereomers in an achiral chromatographic system. The examples of both types of chiral resolution of racemate with using thin-layer chromatography will be presented.