Chromatographic Separations and Analysis: Chiral Separations by Thin Layer Chromatography

Abstract

Racemate separation can be achieved according to two modes. The first mode is direct separation method, and the second is indirect method. The first mode of separation includes the application of a chiral stationary phase (cellulose and its derivatives, CLEC-system, or silica), a stationary phase impregnated with a chiral selector (enantiomers of various chiral compounds, for example acids, alkaloids, amino acids, and their derivatives) or chiral mobile phase additives (e.g., various cyclodextrins and their derivatives, alkaloids). The second mode of separation principle consists of a reaction between a racemate and a chiral derivatizing reagent, which yields separate diastereomers in an achiral chromatographic system. The examples of both types of chiral resolution of a racemate using thin-layer chromatography will be presented.