Abstract
This chapter examines the use of the fluorescence spectroscopy and microscopy in the study of biological systems. The fluorescence technique is attractive since it offers less tedious preparation, high detection sensitivity, and the ability to give spectrotemporal as well as spectrospatial imaging of cells, which allows real-time monitoring of reactive species production in live cells. Detection techniques using fluorescent probes are employed through the aid of optical microscopes, spectrofluorimetric microplate readers, flow cytometric systems allowing high-throughput studies, and high-performance liquid chromatography (HPLC) analysis providing a more specific detection.
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