Abstract
Basic information about instrumentation in HPLC includes presentation of the solvent supply system, pumping system, injectors and autosamplers, analytical columns, detectors and their selection in a HPLC configuration, control and data processing unit. Parameters describing the chromatographic process, retention, and quantitation are included in this chapter. Quantitation in HPLC and the influence of sample volume and analyte amount injected in the chromatographic column on the chromatographic results are detailed. Also, the retention and elution mechanisms in different types of HPLC separation are presented, and the main concepts related to this subject are discussed, such as thermodynamic aspects of partition and adsorption equilibria, the role of polarity in separation mechanisms, and solvophobic theory. The influence of pH, temperature and additives on the retention equilibria are discussed according to the literature focused on these issues.
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