Abstract
The microorganisms that coexist together in an environment are commonly referred to as symbiotic microbes or microbiome. Classically, the symbiotic microorganisms were studied following the culture-based methods but most of the environmental microbes are not cultivable in the laboratory. Thus, the molecular-based methods, especially the high-throughput sequencing technologies (HTS), are adopted to study the symbiotic microbiome. The amplicon and the shotgun metagenomic approaches utilize the HTS technologies for microbiome analyses. While the amplicon analyses help to understand the microbial diversity and abundance, the shotgun metagenomic analyses could reveal the assemblage and functions of microbiome. Today, several options are available for microbiome analyses, but the selection of appropriate workflow is the key to success. This chapter describes the HTS methodologies and bioinformatics workflow available for the functional profiling of symbiotic microbiome. Also, the applications of shotgun metagenomic approach in global microbiome research are discussed. This chapter is aimed to assist the beginners to understand the metagenomic workflow and begin using the metagenomic approach in their research.
Published Version
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