Abstract
Probably the most important technique available to the molecular biologist is DNA sequencing, by which the precise order of nucleotides in a piece of DNA can be determined. The function of a gene can often be deduced from its nucleotide sequence. Initially these techniques were applied to individual genes, but since the early 1990s an increasing number of entire genome sequences have been obtained. Many bioinformatics programs are used during the process of analyzing DNA sequences. Trichoderma species are a rich source of secondary metabolites (SMs) and are considered to be among the most useful fungi in industrial enzyme production. For Trichoderma spp., the estimated genome sizes range from 31 to 39 Mb. The Trichoderma genome contains a multitude of genes encoding biosynthetic pathways for SMs. A Basic Local Alignment Search Tool (BLAST) based method together with phylogenetic information was used to identify and analyze many genes isolated from Trichoderma spp., which had important industrial applications.
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