Abstract
This chapter illustrates isolation and growth of single higher plant cells. Clones of plant tissue were first established from hand-picked single cells of tobacco and marigold callus cultures by Muir, using a nurse culture method. Established cultures of plant cells of many species when grown in a liquid medium on a variety of shaking devices often produce a suspension of single cells and small colonies of cells. The liquid medium is of the same composition as that of the semisolid medium except that the agar is omitted. The shaker or the roller wheel type of agitator thus provides a means for additional aeration and may also loosen the colonies of cells to free single cells. The chapter also discusses that the isolation and growth of single cells and single cell clones of higher plants provide many opportunities to examine cellular details, growth, differentiation, and host cell-environment interactions. Such single cell clones have been useful to clarify biochemical, morphological, and genetic similarities and differences between the normal and diseased plant cells, and to verify totipotency of somatic cells. Many different sizes and shapes of the cells may be observed in the liquid suspension cultures.
Published Version
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