Abstract
Publisher Summary This chapter describes the automation of continuous suspension cultures by the means of a Nephelostat. The Nephelostat is a photocell-controlled continuous culture device, capable of maintaining a steady-state growth in all types of suspension cultures. Light from a tungsten exciter lamp passes through a cylindrical portion of the culture vessel which extends down into the Nephelostat detector assembly. The presence of the cells in the medium produces light scattering, with the consequent excitation of a photocell offset at 30° from the incident light beam. As the cell density increases, the bridge imbalance registers as a positive deflection on a meter on the Nephelostat control console, and this meter reading is also plotted on a strip-chart recorder. The latter provides a continuously recorded analog of cell density. Monitoring of the cell density in this manner occurs at intervals, the frequency of which can be selected by a plug-in timer to accommodate cells with a wide range of growth rates. The Nephelostat can be easily converted to chemostat operation by simply bypassing the nutrient feed control and supplying the nutrient at a constant rate.
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