Abstract
This chapter describes the construction of an amperometric enzymatic sensor for the measurement of glucose. Two coupled enzymes, glucose oxidase and horseradish peroxidase, are used in the sensing phase. They are immobilized by simple dispersion in a carbon paste material, together with ferrocene, which acts as electron transfer mediator for peroxidase. The sensor surface is coated with Nafion to obtain a membrane that prevents losses of the bioreagents and is permeable to small neutral molecules such as glucose. The experiment illustrates a general design for biosensors based on oxidase enzymes, showing high sensitivity and good selectivity under optimized working conditions.
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