Abstract
Transcriptomic analysis using massively parallel cDNA sequencing (RNA-seq) techniques has commonly been used to understand the pathogenesis of various types of pathogens in aquaculture. This technique has contributed enormously and opened up a new strategy to explore the nonmodel organism where the reference genome is lacking. Since the past decade, this technology has been used by several researchers around the world to explore the immune status and understanding of many diseases in fish. Mainly the research emphasized the infectious agent from bacteria and helped us to understand several aspects of the immune response during infection in the fish. This chapter summarizes the workflow and understanding of immune mechanisms in major commercial aquaculture fish species infected by pathogenic bacteria using a transcriptome platform.
Published Version
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