Cadmium Toxicity-Induced Changes in Antioxidative Enzyme Levels in Freshwater Catfish Channa punctatus (Bloch)

Abstract

Present study has been planned to observe the effect of Cadmium chloride on the locally available economically important edible fish _Channa punctatus _(body weight 27.04±0.19 g and length 16.7±0.20 cm). To assess the effect of cadmium in terms of enzymatic and non-enzymatic assays, laboratory studies have been done. Fish were acclimatized and treated separately with concentrations of 35 mg/L and 70 mg/L of CdCl2. The livers were dissected out and homogenized in 0.1 M sodium phosphate buffer (pH 7.4). Biochemical analyses were done for lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and protein estimations. The results were shown as mean and standard error (Mean ± SEM). The analyses of variance (ANOVAs) were analyzed and significantly applied for the Dunnett test for multiple comparisons of groups against the control, which determined the significant differences among the groups. This study is of academic importance and will provide valuable information regarding cadmium toxicity. At the same time, it will address the public health issue as far as consumption of fishes from contaminated water is concerned.