Cadmium Toxicity Induced Changes on Antioxidative Enzymes Level in Fresh Water Catfish Channa Punctatus (Bloch)

Abstract

Aquatic ecosystem of India is being affected by various heavy metals. In present, cadmium contamination in aquatic organism as well as its accumulation in food chain has become a global environmental concern. Cadmium exposure to fishes alter the various enzymatic and histopathologic profiles. Such alteration can be act as a bioindicator for aquatic environment. The present study has been planned to observe the effect of cadmium chloride on commercially important fish _Channa punctatus _(body weight 27.04±0.19 g and length 16.7±0.20 cm). For Invitro assessment of cadmium effect in term of enzymatic and nonenzymatic assay have been done. Fishes were acclimatized and treated separately with different concentration of CdCl2. Dissected out the liver and homogenized in 0.1 M sodium phosphate buffer (pH 7.4). Biochemical analysis was done for lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and protein estimation. The results were shown as mean and standard error (Mean± SEM). The analysis of variance (ANOVA) was analysed and significantly applied for the Dunnett test for multiple comparison of groups against the control which determine the significant differences among the groups. This study is for academic importance and will provide valuable information regarding cadmium toxicity. At the same time, it will address the public health issue as for as consumption of the fishes from contaminated water is concerned.