Bromide-intrusion into Chlorella sp. and Microcystis aeruginosa growing environments: Its impacts on algal growth and the formation potential of algal-derived DBPs upon chlorination

Abstract

Due to the negative impact of climate change and anthropogenic activities, bromide intrusion into algae-impacted freshwater becomes a new challenge for safe drinking water supply worldwide, as bromide and algal organic matter are important disinfection byproduct (DBP) precursors. However, the influences of this phenomenon on algal precursor dynamic and their derived DBPs have to date received little attention. This study examined the effects of bromide intrusion on algal intra- (IOM) and extra-cellular (EOM) precursors during the growth of two freshwater algae Chlorella sp. and Microcystis aeruginosa. Both algae were well-adapted to Br-intrusion, and no significant effect on their growth and their IOM and EOM precursor characteristics was statistically found (p > 0.05). Notwithstanding, this phenomenon apparently added bromide ions into the algal-EOM solution, which resulted in a linear uptake of bromide by IOM. Under Br-intrusion from 0–4 mg/L (Br0–Br4), 15–60% (on average) of the initial bromide additions remained in the algal EOM. By contrast, only an average of ~1.5–2.4% of the additional bromide was taken up by the IOM, resulting in an elevation of brominated DBPs (Br-DBPs) upon chlorination, especially for those samples collected in the late exponential and declined growth phases. When Br0 shifted to Br4, the %Br-DBP yields from both IOM and EOM increased by more than 75%, with a corresponding increasing the total DBP yield of ~30%. The toxic potencies of all chlorinated Br-containing IOM/EOM were thus magnified, by over one order magnitude greater than the non-Br IOM/EOM at Br0. These results are highly significant for understanding the potential risks of Br-intrusion and algal blooming in raw water quality prior to chlorination.