BIOHM Probiotics Retain Viability in Low pH Environments Simulating the Digestive Environment

Ghannoum A,Long L,Ghannoum M,Sun Pl,Isham N

doi:10.35248/2329-8901.7.2.211

Abstract

Probiotics in various combinations and in various forms have become popular supplements for establishing and maintaining gut health. However, commercial products vary in the effectiveness of their specific probiotic strains and the ability of these strains to survive the acidic environment of the stomach. A novel probiotic, manufactured by BIOHM, LLC, has a unique formulation of Saccharomyces boulardii, Lactobacillus acidophilus, L. rhamnosus, and Bifidobacterium breve, in combination with amylase, which has been developed to re-balance the bacterial and fungal population of the human gastrointestinal tract and combat digestive biofilms formed by pathogenic bacteria and fungi. Our data shows that these strains have the ability to survive the acidic environment when taken within 30 minutes of meal, a factor that is vital to ensure probiotic effectiveness.

Highlights

Recent technological advances in DNA sequencing have enabled scientists to identify many of the vast numbers of microorganisms in the human gastrointestinal intestinal (GI) tract
S. boulardii demonstrated no significant difference in average log colony forming units (CFUs)/mL for all pH levels and all time points tested, indicating that S. boulardii suffered no decrease in viability at pH ranges of 5.53-1.5 after 2 hours of exposure
L. rhamnosus demonstrated no significant difference in average log CFUs/mL for pH levels of 5.76-2.5 and all time points tested, indicating that L. rhamnosus suffered no decrease in viability at pH ranges of 5.76-2.5 after 2 hours of exposure

Summary

Introduction

Recent technological advances in DNA sequencing have enabled scientists to identify many of the vast numbers of microorganisms in the human gastrointestinal intestinal (GI) tract. Dysbiosis, or an imbalance in the relative numbers of bacteria and fungi, may lead to a variety of disease states, including inflammatory bowel diseases [2,3,4,5]. In these cases, microbial interactions result in the formation of biofilms, known as digestive plaque, which serve to protect the bacterial-fungal matrix from the effects of therapeutic agents such as antibiotics, as well as from the host immune system [6]

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Discussion

Conclusion