Abstract
The activities and the electrophoretic patterns of ribonuclease (RNase) in leaves of Nicotiana glutinosa plant after mechanical injury, various chemical treatments and/or tobacco mosaic virus (TMV)-inoculation were investigated. The enzyme activity increased and reached a maximum 16hr after mechanical injury. When the injured leaves were inoculated with TMV, the infectivity also showed a maximum value 16hr after mechanical injury. TMV-inoculation induced an increase in the enzyme activity, but the effect was dependent on the inoculum concentration.The enzyme was separated into two isozymes, M and F, by polyacrylamide gel electrophoresis. Mechanical injury and TMV-inoculation of the leaves led to an increase of the activity of M and F isozyme, respectively.Bentonite or sodium dextran sulfate inhibited M isozyme activity, while yeast RNA or eosine Y increased F isozyme activity. However, these chemicals inhibited TMV-infectivity.When the plants were kept at 35C after TMV-inoculation, F isozyme activity was not detected, but a new isozyme band appeared. However, when the same plants were transfered to an incubator maintained at the lower temperature, 21C, the new band was not detected, but F isozyme appeared again.The significance of these results was discussed with respect to a possible role of RNase in the infection of TMV.
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