Abstract
The coumarin compound of osthole was extracted from Cnidium monnieri and identified by LC-MS and 1H- and 13C-NMR. Osthole was tested for anti-virus activity against tobacco mosaic virus (TMV) using the half-leaf method. The results showed that stronger antiviral activity on TMV infection appeared in Nicotiana glutinosa than that of eugenol and ningnanmycin, with inhibitory, protective, and curative effects of 72.57%, 70.26%, and 61.97%, respectively. Through observation of the TMV particles, we found that osthole could directly affect the viral particles. Correspondingly, the level of coat protein detected by Western blot was significantly reduced when the concentrations of osthole increased in tested plants compared to that of the control. These results suggest that osthole has anti-TMV activity and may be used as a biological reagent to control the plant virus in the half-leaf method.
Highlights
Tobacco mosaic virus (TMV) belongs to the genus Tobamovirus and is transmitted by mechanical inoculation and insects with chewing mouthparts in a propagative manner [1,2]
We investigated whether the exogenous application of osthole is able to induce anti-viral activity in the tobacco plant when infected with tobacco mosaic virus (TMV)
TMV coat protein (CP) accumulation detected by Western blot analysis
Summary
Tobacco mosaic virus (TMV) belongs to the genus Tobamovirus and is transmitted by mechanical inoculation and insects with chewing mouthparts in a propagative manner [1,2]. Many kinds of plant compounds have already been demonstrated to have anti-viral ability, such as Amaranthaceae, Nyctaginaceae, Asteraceae, Chenopodiaceae, Asclepiadaceae, Polygonaceae, Simaroubaceae, Acanthaceae, Liliaceae, Cruciferae, Leguminosae sp., Boraginaceae, Oleaceae, Taxaceae, Ranunculaceae, Juglandaceae, Saxifragaceae, Theaceae, Schisandraceae, Cupressaceae, Labiatae, and Caryophyllaceae [12,13,14,15]. Among these plants, the effective antiviral compounds are mainly proteins, alkaloids, flavonoids, phenols, essential oils, and polysaccharides. We observed whether osthole could affect the TMV particles and coat protein (CP) accumulation
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