Automated Assessment of Loss of Consciousness Using Whisker And Paw Movements During Anesthetic Dosing in Head-Fixed Rodents.

Abstract

The precise identification of loss of consciousness (LOC) is key to studying the effects of anesthetic drugs in neural systems. The standard behavioral assay for identifying LOC in rodents is the Loss of Righting Reflex (LORR), assessed by placing the animal in the supine position every minute until it fails to right itself. However, this assay cannot be used when the rodents are head-fixed, which limits the use of powerful techniques such as multi-electrode recordings, in vivo patch clamp, and neuronal imaging. In these situations, an alternative way to assess LOC is needed. We propose that loss of movement (LOM) in whiskers and paws of head-fixed animals can be used as an alternative behavioral assay in head-fixed animals. Unlike LORR, LOM in whiskers and paws is much harder to detect by visual inspection. Therefore, we developed a method to automatically assess for LOM of whiskers and paws in head fixed rodents during in vivo patch clamp recordings. Our method uses an algorithm based on optical flow and point-process filtering which can be run on images acquired on regular cameras at low frame-rates. We show that the algorithm can achieve at least comparable accuracy in detecting LOC when compared with consensus among human observers, as well as improved precision when compared with individual observers. In the future, we aim to to expand the method to detect more behavioral end-points during anesthesia such as paradoxical excitation. Eventually, we hope to enable multi-modal anesthesia studies, which incorporates behavioral and neurophysiological data.