Association between inducible nitric oxide synthase gene polymorphism and ischemic cardio-cerebrovascular disease in Chinese Han population

Abstract

Objective To evaluate the association between inducible nitric oxide synthase (iNOS) gene polymorphisms and ischemic cardio-cerebrovascular disease in Chinese Han population. Methods Two independent case-control studies were conformed, including 291 cases of coronary heart disease (CHD) and 487 healthy subjects, 246 cases of ischemic stroke and 246 healthy subjects. The genotype of rs2779248(C/T) and rs1137933(C/T) were determined by Taqman single nucleotide polymorphism (SNP) genotyping assays. Results T allele of rs2779248 and rs1137933 may increase the risk for CHD or ischemic stroke in Chinese Han population. combined with the two independent case-control studies, the risk relationship also existed. T allele had higher risk for ischemic cardio-cerebrovascular disease than C allele with the odds ratio being 1.33 [95% confidence interval (CI): 1.09-1.61] for rs2779234, and 1.42 (95%CI: 1.16-1.73) for rs1137933, respectively. Adjusted by conventional risk factors, rs2779248 TT genotype still was a risk factor for ischemic cardio-cerebrovascular disease [odds ratio (OR)=2.75, 95%CI: 1.38-5.49]. As compared with CC genotype, individual with CT+ TT genotype of rs1137933 had higher risk with the odds ratio being 1.37 (95%CI: 1.03-1.82). Conclusion Our study revealed the polymorphisms of rs2779248 and rs1137933 in iNOS gene were associated with ischemic cardio-cerebrovascular disease in Chinese Han population. Key words: Inducible nitric oxide synthase gene; Ischemic cardio-cerebrovascular disease; Genetic polymorphism; Risk factors