Correlation between inducible nitric oxide synthase gene polymorphism and ischemic stroke in Uygur population of Xinjiang China

Abstract

Objective To explore the association between inducible nitric oxide synthase (iNOS) gene polymorphisms and ischemic stroke in Uygur population of Xinjiang. Methods A case-control study was performed, included 316 patients with ischemic stroke, admitted to our hospital from June 2007 to December 2013, and 316 age- and gender-matched healthy subjects in Uygur population of Hami. The genotypes of rs2779248(C/T) and rs1137933(C/T) were determined by Taqman probe fluorescent quantitative PCR. Results The distribution frequencies of rs2779248 (CC, CT and TT) were 51.9%, 42.1% and 6.0% in the patient group, ands 61.7%, 30.1% and 8.2% in the controls, respectively. As compared with those with rs2779248 CC genotype, individuals with CT+TT genotype and CT genotype had higher risk of ischemic stroke, the odds ratio was 1.49 (95%CI: 1.09-2.05, P=0.013) and 1.66 (95%CI: 1.19-2.32, P=0.003). The distribution frequencies of rs1137933 (CC, CT and TT) were 66.8%, 28.5% and 4.7% in the patient group, ands 66.8%, 27.8% and 5.4% in the controls, respectively; no significant difference was noted between the patient group and controls in the distribution frequencies (P>0.05). Multi-factor Logistic regression analysis indicated that mutation of genotype C>T in rs2779248 was the independent risk factor of ischemic stroke in Uygur population of Hami after adjusting related risk factors (OR=1.55, 95%CI: 1.00-2.40, P=0.049). Conclusion The polymorphisms of rs2779248 in iNOS gene may modify the risk of ischemic stroke in Uygur population of Hami, but no association is found in rs1137933. Key words: Inducible nitric oxide synthase; Ischemic stroke; Genetic polymorphism; Risk factor; Uygur population