Abstract
Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.
Highlights
Lots of assays are routinely used to assess hair growth, while hair follicle organ culture model is one of the most popular and influential in vitro systems [1]
In the untreated test item group, the follicles were restored their integrity to some extent with slightly increased the thickness of hair bulb by 20.9% and 28.2% on day 5 and 7, respectively concerning day 1 (Figure 1)
Follicles were observed to have catagen-like changes with increased in hair bulb thickness in both minoxidil and untreated test item groups (Figure 3A)
Summary
Lots of assays are routinely used to assess hair growth, while hair follicle organ culture model is one of the most popular and influential in vitro systems [1]. Rodent vibrissa follicles are regular, predictable, and relatively short growth cycles Considering these properties, authors choose mouse vibrissa follicles as a test model for the assessment of hair cycle and compared morphologic changes in culture [2]. Complementary and Alternative Medicine (CAM) therapies are rising as preferred models of treatment, among which Biofield Therapy (or Healing Modalities) is one approach that has been reported to have several benefits to enhance physical, mental and emotional human wellness. CAM therapies have been practiced worldwide with reported clinical benefits in different health disease profiles [9]. This energy can be harnessed and transmitted by the experts into living and non-living things via the process of Biofield Energy Healing. Biofield Energy Treatment (The Trivedi Effect®) has been published in numerous peer-reviewed science journals with significant outcomes in many scientific fields such as cancer research [10, 11], microbiology [12-15], biotechnology [16, 17], pharmaceutical science [18-21], agricultural science [22-25], materials science [26-29], nutraceuticals [30, 31], skin health, human health and wellness
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