Abstract
The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.
Highlights
Our planet is facing an energy crisis as a result of the use of nonrenewable and limited energy resources (Doolotkeldieva and Bobusheva, 2011; Amin and Rahman, 2019)
Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material
Most of the isolates revealed ability to carboxymethyl cellulose (CMC) degradation, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity (FPA), EGs and BGLs activities (p
Summary
Our planet is facing an energy crisis as a result of the use of nonrenewable and limited energy resources (Doolotkeldieva and Bobusheva, 2011; Amin and Rahman, 2019). Trichoderma genus (Persoon, 1794) comprises an important number of many saprotrophic species with a wide ecological distribution. They are capable of growing on soil, bark, wood, other fungi and inother types of substrates demonstrating their opportunistic potential and their adaptability to different ecological conditions (Druzhinina et al, 2011). Many members in Trichoderma genus are key microorganism in biotechnologically due to their ability to secrete a wide spectrum of enzymes and bioactive compounds (Mandels and Reese, 1957; Doolotkeldieva and Bobusheva 2011; Druzhinina et al, 2011). This work includes: (a) the isolation of new strains of Trichoderma searching for optimal enzyme secretion; (b) the analysis of the cellulase secretion of these strains by means of qualitative and quantitative methods; (c) the evaluation of the congruence between the qualitative and quantitative results; (d) the selection of the Trichoderma isolate with the highest
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