Abstract
The cellulase production byPenicillium decumbens L-06 in solid state fermentation (SSF) was investigated using bagasse as the substrate in this paper. The optimum conditions for cellulase production achieved by single factor testing were: the ratio of bagasse to wheat bran 1∶1 (w/w), the ratio of water to material 3∶1 (v/w), culture temperature 30 °C, initial pH 5.0, ammonium sulphate as nitrogen source with the concentration of 1%, 6 day’s fermentation period. BoxuBehnken factorial design (BBD) and response surface methodology (RSM) were further used to optimize conditions for cellulase (Filter paper activity) production. The maximal cellulase (Filter paper activity) production (3.89 FPu g−1) was obtained under the optimized conditions (ratio of water to material 2.38∶1, initial pH 5.28, cultivation time 150.5 h). It was well corresponded to the calculated results (3.97 FPu g−1) by model prediction.
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