Abstract
Bacteria are smallest primitive, simple, unicellular, prokaryotic and microscopic organisms. But these organisms cannot be studied with naked eyes because of their minute structure. Therefore in search for the information about the structure and composition of bacterial cells, cell biologist used light microscopes with a numerical aperture of 1.4 and using wavelength of 0.4 μm separation. But there are still certain cellular structures that cannot be seen through naked eyes, and for them electron microscope is used. There are certain improved types of light microscope which can be incorporated to improve their resolving power. Hence microscopy is playing a crucial role in the field of bacteriology.
Highlights
To get acquainted with the world of bacteria like small organisms, very effective and advanced technique is required
There are more than thousand species of bacteria, but all of them are basically of three different shapes, some are like little balls and called cocci, others are still helical or spiral in shape, like Borrelia, Some bacterial cells exist as individuals while others cluster together to form pairs, chains, squares or other groupings
There are various microscopic techniques which are employed in the field of bacteriology, for particular morphology, anatomy and physiology and various shapes and sizes of bacterial strains which is the basis for the identification of a particular bacteria and diagnosing diseases caused by pathogenic species
Summary
To get acquainted with the world of bacteria like small organisms, very effective and advanced technique is required. Microscopy promises to be a valuable tool for diagnosing and measuring the bacterial cell surfaces. The technical concept of using microscopes for the study of such small samples like bacteria etc. Microscopy proved as a boon to find and identify causal bacteria and analyze samples to diagnose disease in organism. In this chapter we have reviewed about different microscopic techniques to study morphological as well as physiological characteristic of various bacterial strains. Atomic force microscopy overcame the challenge of sample preparation by using contact mode—AFM to study cell growth and division of AFM [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
