Antioxidant Activity of Sunflower (Helianthus Annuus l.) Ethanolic Extract with DPPH Method and Determination of Total Phenolic and Flavonoid Levels

Salwa Salwa

doi:10.23917/jnhm.v4i1.15642

Abstract

Sunflower (Helianthus annuus L.) is believed to have antioxidant properties. Currently, there are many studies examining compounds that have benefits as antioxidants and are safe for health. However, the sunflower only examined the antioxidants in sunflower seeds. This study aims to determine the antioxidant activity of ethanol extract of flowers, bark, and leaves of sunflowers. The antioxidant activity test using the DPPH method showed the highest antioxidant activity was found in the leaf extract (Helianthus annuus L.) with an IC50 value of 48.841 ppm. The highest phenolic content was found in leaf samples with a value of 35.149 mg gallic acid equivalent/gram extract and the highest determination of flavonoid levels was found in leaf samples with a value of 10.917 mg quercetin equivalent/gram extract.

Highlights

Antioxidants can stabilize or deactivate free radicals before they attack cells, so the risk of degenerative diseases such as cardiovascular, atherosclerosis, and other degenerative diseases can be reduced by consuming adequate amounts of antioxidants (Sayuti and Yenrina, 2015).The sunflower plant has many benefits, one of which is as an antioxidant (Dwivedi, 2014)
The article written by Ali (2014) stated that the ethanolic extract of sunflower seeds had higher antioxidant activity, and (Juniarti & Herdiana, 2003) explained that all parts of the sunflower plant have antioxidant, antitumor, and antimicrobial activities
The materials used consisted of flowers, leaves, the bark of sunflower plants (Helianthus annuus L.), 96% ethanol, absolute ethanol DPPH, aquadest, aluminum foil, N2CO3 7.5%, Folin-Ciocalteu 10%, gallic acid AlCl3 10%, potassium acetate 1M, quercetin

Summary

Introduction

Antioxidants can stabilize or deactivate free radicals before they attack cells, so the risk of degenerative diseases such as cardiovascular, atherosclerosis, and other degenerative diseases can be reduced by consuming adequate amounts of antioxidants (Sayuti and Yenrina, 2015).The sunflower plant has many benefits, one of which is as an antioxidant (Dwivedi, 2014). Antioxidants can stabilize or deactivate free radicals before they attack cells, so the risk of degenerative diseases such as cardiovascular, atherosclerosis, and other degenerative diseases can be reduced by consuming adequate amounts of antioxidants (Sayuti and Yenrina, 2015). Several reports on research on sunflower plants, including methanol extract of sunflower seeds (Helianthus annuus L.) is known to contain phenolic compounds such as tannins, alkaloids, and flavonoid (Dwivedi, 2014). The ethanol extract of sunflower leaves has antioxidant, antidiarrheal, and antihistamine activity (Dwivedi and Sharma, 2015). The article written by Ali (2014) stated that the ethanolic extract of sunflower seeds had higher antioxidant activity, and (Juniarti & Herdiana, 2003) explained that all parts of the sunflower plant have antioxidant, antitumor, and antimicrobial activities

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Conclusion