An Insight into Nano Silver Fluoride-Coated Silk Fibroin Bioinspired Membrane Properties for Guided Tissue Regeneration.

Aditi Pandey,Aivaras Kareiva,Ching-Shuan Huang,Jen-Chang Yang,Nai-Chia Teng,Wendimi Fatimata Belem,I-Wen Chen,Tzu-Sen Yang,Ta-I Yang

doi:10.3390/polym13162659

Aditi Pandey, Aivaras Kareiva + Show 7 more

Open Access

https://doi.org/10.3390/polym13162659

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Abstract

The current work focuses on the development of a novel electrospun silk fibroin (SF) nonwoven mat as a GTR membrane with antibacterial, biomineralization and biocompatible properties. The γ-poly glutamic acid (γ-PGA)-capped nano silver fluoride (NSF) and silver diamine fluoride (SDF) were first synthesized, which were dip-coated onto electrospun silk fibroin mats (NSF-SF and SDF-SF). UV-Vis spectroscopy and TEM depicted the formation of silver nanoparticles. NSF-SF and SDF-SF demonstrated antibacterial properties (against Porphyromonas gingivalis) with 3.1 and 6.7 folds higher relative to SF, respectively. Post-mineralization in simulated body fluid, the NSF-SF effectively promoted apatite precipitation (Ca/P ~1.67), while the SDF-SF depicted deposition of silver nanoparticles, assessed by SEM-EDS. According to the FTIR-ATR deconvolution analysis, NSF-SF portrayed ~75% estimated hydroxyapatite crystallinity index (CI), whereas pure SF and SDF-SF demonstrated ~60%. The biocompatibility of NSF-SF was ~82% when compared to the control, while SDF-coated samples revealed in vitro cytotoxicity, further needing in vivo studies for a definite conclusion. Furthermore, the NSF-SF revealed the highest tensile strength of 0.32 N/mm and 1.76% elongation at break. Therefore, it is substantiated that the novel bioactive and antibacterial NSF-SF membranes can serve as a potential candidate, shedding light on further in-depth analysis for GTR applications.

Highlights

The component used for the construction of the membrane used for guided tissue regeneration (GTR) is a vital aspect of its efficacy
No work has been reported for the application of nano silver fluoride (NSF)/silver diamine fluoride (SDF)-coated materials in the field of guided tissue regeneration. Considering these facts, this work aims for the development of an NSF-coated electrospun silk fibroin (SF) and compared it with SDF-SF
The extracts from NSF-SF support the cell growth this study is 38% (CSDF) and 1% (DSDF), which has a higher concentration of Ag, th, despite the presence of nano silver

Summary

Introduction

Periodontium regeneration can be achieved by various clinical therapies opted for treating severe periodontitis lesions. Periodontal defect reconstruction via guided tissue regeneration (GTR) membranes is one of the successful surgical techniques to resist proliferating connective tissue from migrating into the defect site [3]. The component used for the construction of the membrane used for GTR is a vital aspect of its efficacy. The membrane should fulfill the main designing criteria for GTR, such as biocompatibility, cell occlusion, spaciousness, clinical manageability, and tissue integration [4]. Regarding the membrane materials used, various examples include synthetic polymers such as polytetrafluoroethylene (PTFE), polylactic acid (PLA), polyglutamic acid (PGA), natural polymers such as collagen, chitosan, gelatin, etc. Regarding the membrane materials used, various examples include synthetic polymers such as polytetrafluoroethylene (PTFE), polylactic acid (PLA), polyglutamic acid (PGA), natural polymers such as collagen, chitosan, gelatin, etc. [5,6]

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