Abstract
A simple and accurate method has been devised for the quantitative analysis of protein hydrolysates using thin-layer chromatography. Ninhydrin—cadmium acetate is the staining reagent and, using reflectane densitometry, calibration graphs have been drawn for twenty-two naturally occurring amino acids. The equation of the line for each amino acid has been calculated and used for the determination of the amount of amino acid present in protein hydrolysates. The results obtained agree well with those obtained by the ∝Technicon’ Automatic Amino Acid Analyzer and with those given in the literature.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.