An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose : II. The quantitative determination of amino acids in protein hydrolysates

Abstract

A simple and accurate method has been devised for the quantitative analysis of protein hydrolysates using thin-layer chromatography. Ninhydrin—cadmium acetate is the staining reagent and, using reflectane densitometry, calibration graphs have been drawn for twenty-two naturally occurring amino acids. The equation of the line for each amino acid has been calculated and used for the determination of the amount of amino acid present in protein hydrolysates. The results obtained agree well with those obtained by the ∝Technicon’ Automatic Amino Acid Analyzer and with those given in the literature.