An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose : Part IV. The quantitative determination of amino acids in urine

Abstract

A new method is described for the accurate quantitative determination of amino acids in urine using thin-layer chromatography. Interfering salts and peptides are first removed from the urine by passing it through a column of an ion-retardation resin. The urine is then chromatographed on thin layers of cellulose and the amino acids are determined quantitatively by the method of H eathcote and H aworth. Several normal and pathological urines have been examined by this technique and quantitative recovery of standard amino acids has been obtained. The results obtained with individual urine samples agree well with those determined by the automatic ion-exchange method of analysis. The method seems to hold considerable promise for the analysis of amino acids in urine.