Quantitative determination of amino acids in protein hydrolyzates by thin-layer chromatography

Abstract

A micro method for the quantitative determination of amino acids in protein hydrolyzates has been proposed which permits the analysis of two hydrolyzates to be performed in 4–5 h. After the separation of the amino acids by TLC in cellulose, they are determined quantitatively by the colorimetric method using a MKMF-1 microphotocolorimeter. Proline and tryptophan are determined separately with the aid of specific test-tube reactions. The sensitivity of the method permits the analysis of 10–15 µg of aminoacid mixture.