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Abstract
Summary An improved method for the quantitative assay of complex amino acid mixtures has been described. Complete separation of amino acids in simple protein hydrolyzates has been achieved by one-dimensional paper chromatography using a number of alcohol solvents. Direct photometric scanning of these one-dimensional chromatograms subjected to a new modification of the ninhydrin reaction (or isatin reaction in the case of proline) is the basis of the assay. The coefficient of variation for a single determination is ±4.36%. The analysis of bovine serum albumin and of a synthetic mixture of amino acids has been reported.
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