Acute Effects Of Diazinon On Blood Paramters In The African Catfish (<italic>Clarias Gariepinus</italic>)

Abstract

The aim of this study was to assess the effect of diazinon (0,0-diethyl 0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate) on cultured and wild African catfish (Clarias gariepinus).The effect was assessed based on results of acute toxicity tests and on a comparison of results of haematological examination of a control and an experimental group exposed to Diazintol R pesticide preparation (active substance 162mg.l -1 showed significantly lower values (p < 0.05) of erythrocyte count (RBC), haemoglobin content (Hb) and haematocrit (PCV) compared to the control group. Values of MCV, MCH and MCHC were comparable in both groups during the study. In contrary, there was a significant decrease in leucocyte count (p < 0.05), as well as in both the relative and absolute lymphocyte count (p < 0.05) and a significant increase in both the relative and absolute count of developmental forms of neutrophile granulocytes: myelocytes (p < 0.05) and metamyelocytes (p < 0.05) in the experimental group. Relative and absolute count of monocytes and both the band- and segmented neutrophile granulocytes was comparable in both groups during the study. Changes in values of both the erythrocyte and leucocyte profile after exposure to diazinon-based preparation may be referred to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish.