Abstract 4759: Nanoscale Huangjing extract from Mount Tai displays enzyme and biofuel enhancing activity: Potential in precision anti-cancer therapy

Abstract

Abstract Chinese herb Huangjing has been well-known for its anti-cancer characters in xenograft models of cancers and traditional Chinese herbal classical books. However, the mechanisms are largely unknown. The processed Huangjing has the pharmacological function but fresh forms may have toxicity. Mount Tai is famous for its traditional culture and Chinese medicine resources with special environmental and nutritional potential for enrichment of herbal chemical competent. Our study aimed to test phosphatase activity of nanoparticles produced in the process of herb and to identify whether its anti-cancer properties is related to the phosphatase activity. Phosphatase has been known for its function in dephosphorylating of many drug targets therein to inhibit cancer cell growth. We recently reported engineered nanoparticles were proved to exhibit phosphatase activity and here we further demonstrated that nanoscale Huangjing herb from Mount Tai showed positive results on a few phosphatase substrates. The corresponding substrate specificity and kinetics of the reaction including the substrate concentration, pH, and temperature dependence were studied in detail. In addition, using a Biorad Biofeul kit, Huangjing was observed to catalyze p-nitrophenyl glucopyranoside to p-nitrophenol reaction. Our data suggest that Huangjing may cleave the covalent bond between beta glucose and nitrophenol suggesting the biofuel-like activity. We then performed experiments to test the effectiveness of Huangjing at nanoscale for inhibiting growth of cancer cells wherein, the growth of MDA-MB-231 and PC3 cells was greatly inhibited while normal cells are planning for comparison and investigation in mechanisms. The study has a significance in developing potential novel nanoscale drugs with phosphatase-like activity for treatment of certain cancers with combination of targeted kinase inhibitors based therapy or as a drug delivery machinery. Our preliminary results suggest a kinase synergistic based signaling. Thus, our data provided the potential concept of the combination of traditional Chinese medicine with modern chemotherapy. Citation Format: Aigerim Kabulova, Madina Razbekova, Yingqiu Xie, Adilet Dautov, Qian Wang, Haiyan Fan. Nanoscale Huangjing extract from Mount Tai displays enzyme and biofuel enhancing activity: Potential in precision anti-cancer therapy [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 4759.