A Study of the Genetic Diversity of Globulin Proteins in Lens culinaris Medik. Genotypes

Abstract

Electrophoretic analysis of globulin storage proteins in the seeds of 46 lentil accessions introduced from ICARDA was carried out. The aim of the work was to identify, certification and study the genetic diversity of lentil genotypes. In addition, a genetic diversity index (H) was calculated for the zones (ω-, γ-, β- and α-) based on the frequency of patterns on electropherograms of spare globulin proteins in seeds of lentil accessions. Twenty-two spectra and 55 patterns were identified in lentil accessions, most of which polymorphism was observed. 7 spectra and 22 patterns were observed in the ω-zone, 7 spectra and 9 patterns in the γ-zone, 5 spectra and 11 patterns in the β-zone, and 5 spectra and 13 patterns in the α-zone. The genetic diversity index was calculated for each of the 4 zones (ω, γ, β and α) based on Nei formula. According to the calculations, more genetic diversity was observed in ω-zone (H=0.930), slightly less in β-zone (H=0.872), α (H=0.827) and the least in γ-zone (H=0.743). Based on cluster analysis, the genotypes were divided into 5 groups and subgroups. Based on these results, an electrophoretic analysis of globulin storage proteins in polyacrylamide gel (A-PAAG) was performed for the first time and polymorphism between lentil genotypes was identified.